How to Prepare Samples:
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Step 1
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Prepare up to 2 mg total protein sample for loading.
Prepare a Minigel or standard SDS-PAGE gel using 1 mm thickness spacers
and a prep-comb (with 1 big flat tooth) to
make a wide flat well, deep enough to meet the loading volume.
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Step 2
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Run the gel as regular SDS-PAGE procedure to separate the protein of interest.
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Step 3
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Stain the gel with coomassie blue.
Destain the gel and identify the protein band of interest.
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Step 4
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Cut the band of interest using the whole width of the gel
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Step 5
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Transfer the gel strip with protein band into a tube, and send the sample for antibody production. The sample can be sent at room temperature.
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