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DAB HRP Substrate Kit for IHC


dab303 imageDAB HRP Substrate Kit for IHC (Cat. No. DAB-303) A high performance DAB (3,3'-Diaminobenzidine) liquid chromogen substrate kit, recommended for use in HRP- based IHC staining procedures. Supplied as a two-component system, this DAB detection reagent kit has two solutions (Solution A and Solution B) required for HRP chromogen reaction. Solution A contains the substrate buffer and H2O2, and Solution B contains stablized liquid DAB chromogen in concentrated stock.

This DAB liquid chromogen substrate kit offers a sensitive and rapid clean detection performance in IHC stainings. In the presence of peroxidase and hydrogen peroxide, DAB chromogen is oxidized to produce alcohol-insoluble dark brown color precipitate and deposit at the HRP conjugate targeted site.


  • Immunohistochemistry (IHC)
  • In Situ Hybridization (ISH)

Supplied AsTwo-component system, Solution A: HRP substrate buffer and H2O2 (1X); Solution B: DAB chromogen substrate (30X)
PrecautionsThis product contains DAB: a potentail CARCINOGEN which should be handled by professional users only. MSDS is available for users on request
Intended UseFor Research Use or Manufacturing Purpose Only
StorageStore at 2-8 °C for up to 12 months from the date received.
ShippingShipped at ambient temperature.
Warranty/ConditionsGeneral Bioscience products may not be resold or modified for resale without prior written approval.

Ordering Information

DAB-303-77 ml$95
DAB-303-1515 ml$160

Detailed ordering information is shown on the How to Order web page (page link is located in the upper-right area of this page).

Kit Components

  • Solution A (30X): HRP substrate buffer and Hydrogen Peroxide
  • Solution B (30X): HRP DAB chromogen substrate

Data Sheet (PDF)
Order Form (PDF)

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