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Chemiluminescence Detection Kit

Description


ecl401 imageChemiluminescence Detection Kit (Cat. No. ECL-401) The enhanced chemiluminescence detection reagents is recommended for use in horseradish peroxidase (HRP)-based Western Blotting and ELISA immunodetection procedures. Provided as a two-component system, this kit contains Solution A (HRP substrate buffer and H2O2) and Solution B (stablized chemiluminescent luminol and enhancers).


In the presence of peroxidase, the reagents emits chemiluminescent light. The chemiluminescent light emitting can be quantitatively detected via regular autoradiograph film, CCD camera, or chemiluminescence reading device. In immunoblotting procedure, this reagent kit offers a clean detection performance on PVDF and nitrocellulose membrane blots.

Applications

  • Western Blotting
  • ELISA


Supplied AsTwo-component reagent kit, Solution A: HRP substrate buffer and H2O2 (1X), Solution B: chemiluminescence luminol and enhancer (40X)
PrecautionsThe working mixture of two reagents is stable for serveral hours when protected from light. MSDS is available for users on request.
Intended UseFor Research Use or Manufacturing Purpose Only
StorageStore at 2-8 °C. This product is light-sensitive, should be protected from sunlight or UV light
ShippingShipped at ambient temperature.
Warranty/ConditionsGeneral Bioscience products may not be resold or modified for resale without prior written approval.


Ordering Information


CAT. NO.SIZE/QTYPRICE (USD)
ECL-401110 ml$62

Detailed ordering information is shown on the How to Order web page (page link is located in the upper-right area of this page).



Kit Components

  • Solution A (1X): HRP substrate buffer and Hydrogen Peroxide.
  • Solution B (40X): HRP Luminol Substrate and Enhancer


DOCUMENTS
Data Sheet (PDF)
Order Form (PDF)



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