TMB OneSolution HRP Substrate for ELISADescription
 TMB OneSolution HRP Substrate for ELISA (Cat. No. TMB-001) This ready-to-use TMB (3,3',5,5' -tetramethybezidine) substrate reagent is recommended for use in HRP-based immunoassay procedures. With the optimized formulation of stablized TMB, substate buffer and H2O2, this TMB reagent contains all essential components in one solution, and offers a sensitive and clean detection performance.
When HRP enzyme reaction occurs, this TMB solution produces a blue color reaction product. The enzyme reaction may be stopped after 5-10 minutes by adding diluted sulfuric acid, resulting the blue color changes to yellow color which is stable for several hours. Quantitative measurement should be carried out using microplate reader. The absorbance of yellow color should be measured at 450nm or 405nm. Without stopping step in the reatction, the blue color may be read at 655nm.
| Supplied As | Ready-to-use reagent solution |
| Precautions | This product should be handled by trained users only, avoid contact with eyes and skin. MSDS is available for users on request. |
| Intended Use | For Research Use or Manufacturing Purpose Only |
| Storage | Store at 2-8 °C, stable for 12 months from the date received |
| Shipping | Shipped at ambient temperature. |
| Warranty/Conditions | General Bioscience products may not be resold or modified for resale without prior written approval. |
Ordering Information
| CAT. NO. | SIZE/QTY | PRICE (USD) |
| TMB-001-100 | 100 ml | $50 |
| TMB-001-500 | 500 ml | $145 |
| TMB-001-1L | 1 liter | $240 |
| TMB-001-C | bulk qty | inquire |
Detailed ordering information is shown on the How to Order web page (page link is located in the upper-right area of this page).
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