Home » hrp substrates » tmb elisa substrate

TMB OneSolution HRP Substrate for ELISA

Catalog Number: TMB-001
Product IDSizePrice (USD)
TMB001-100100 ml$70.00
TMB001-500500 ml$195.00
TMB001-1L1 Liter$240.00
ShippingShipped at ambient temperature
StorageStore at 2-8 °C, the reagent is stable for at least one year after received
Intended UseFor Research Use Only


This ready-to-use reagent contains all essential components (TMB, substate buffer and H2O2) in one solution for HRP-based ELISA. The TMB OneSolution is stable, and offers high sensitivity and consistent performance.

TMB (3,3',5,5' -tetramethybezidine) is commonly used in HRP-based ELISA (emzyme-linked immunosorbent assay). This TMB reagent is formulated as all-in-one, ready to use HRP substrate. When HRP enzyme reaction occurs, the substrate reagent produces a blue color reaction product. Normally, the enzyme reaction should be stopped after 5-10 minutes by adding diluted sulfuric acid, resulting a change to yellow color which is stable for optical density adsorbance reading. Quantitative measurement should be carried out using microplate reader. The absorbance of yellow color should be measured at 450nm or 405nm. Without stopping step in the reatction, the blue color may be read at 655nm.

  • The order (from inside USA) can be shipped out on the same day before 1:00 pm pacific time.
  • Most items are usually shipped on wet-ice pack through cost-effective services (such as FedEx 2-days, UPS).
  • Overnight shipping is available upon request.
  • The shipment tracking will be provided via email.
× Categories