TruVisionTM Poly-HRP IHC Detection Kit (anti-rabbit with DAB)HIGHLIGHTS
- Outstanding sensitivity and clean detection signal
- No need for biotin-blocking step
- Easy-to-use, with a simplied procedure
Description
TruVisionTM Poly-HRP IHC Detection Kit (anti-rabbit with DAB) (Cat. No. IHC-201) The TruVisionTM IHC Detection System is based on the site-directed clustering/coupling chemistry by which Poly-HRP conjugates are created as polymerized peroxidase clusters attached to detection antibodies. The polymerized peroxidase clusters on detection antibody offers outstanding sensitivity and signal intensity. This novel approach employed to prepare enzyme-labeled secondary antibodies avoids addition of linear polymer backbone molecules or biotin-avidin complex to reduce non-specific bindings in IHC staining. The cluster nature of polymerized peroxidase allows better permeation of the reagents to the target sites in tissue sections.
This TruVisionTM IHC detection kit is designed for use with rabbit primary antibodies to detect the target protein markers in human tissue sections. The reagents are supplied in a convenient dropper bottle format, as ready-to-use blocking reagent and Poly-HRP conjugate, and concentrate DAB chromogen substrate, Solution A (30X) & Solution B (30X).
Applications- Paraffin-embedded human tissue sections (primary antibody: rabbit IgG)
- Cryostat human tissue sections (primary antibody: rabbit IgG)
| Supplied As | Ready-to-use reagents and concentrate two-component DAB kit |
| Intended Use | For Research Use Only |
| Storage | Store at 2-8 °C |
| Shipping | Shipped at ambient temperature. |
| Warranty/Conditions | General Bioscience products may not be resold or modified for resale without prior written approval. |
Ordering Information
| CAT. NO. | SIZE/QTY | PRICE (USD) |
| IHC-201-15 | 15 ml | $270 |
| IHC-201-110 | 110 ml | $700 |
Detailed ordering information is shown on the How to Order web page (page link is located in the upper-right area of this page).
Kit Components
- Blocking Reagent, ready-to-use
- Poly-HRP Anti-rabbit IgG Conjugate, ready-to-use
- DAB Solution A: HRP substrate buffer (30X)
- DAB Solution B: stablized DAB chromogen solution (30X)
- User Instruction
- Size 15 ml: sufficient for 150 slides; size 110 ml: for 1100 slides (based on 0.1 ml per section)
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