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Phospho-Specific Polyclonal Antibody Production
ES905: Phospho-Specific Antibody Production & Purification, Complete Package (rabbit)
Includes:
- Peptide synthesis for phosphorylation modification (<15 a.a. >90% purity, 15 mg)
- Peptide synthesis for non-modified peptide (<15 a.a. >70% purity, 10 mg)
- Conjugation to KLH (a cysteine is automatically added to either C-term or N-term of peptide)
- 2 rabbits, pre-immune bleed, 4 immunizations for each rabbit
- Production bleeds, 100-120 ml antiserum from two rabbits
- Two affinity columns: phospho-peptide column (5 mg phospho-peptide) and non-modified peptide column
- Phospho-peptide affinity purification and peptide cross-adsorption process
- ELISA test
- 3 ml of pre-immune serum
- Phospho-specific antibodies from two rabbits
- ELISA result
- 5 mg of phospho-peptide, 5 mg of non-modified peptide, MS and HPLC reports
- Total service turnaround: 15-17 weeks
